Ultra-sensitive FRET CST Biosensor for Early and Non-invasive Diagnosis of Alzheimer's Disease

# Record card
319
Thematic areas
Health & Biotech
Health & Biotech / Diagnostic kits
Health & Biotech / Diagnostic, Medical imaging & advanced bioimaging
Health & Biotech / Biosensors
Description

The technology is an ultra-sensitive optical biosensor based on the FRET principle, designed for identifying the initial, pre-aggregated pathological form of the Tau protein (CST). The innovation lies in the ability to detect specific conformational changes (CST), providing a molecular advantage over concentration-only tests.

In vitro validation (TRL 3) has been successfully completed, demonstrating the sensor's effectiveness on:

  1. Human brain lysates containing pathological Tau.
  2. Identification of responses to drugs and synthetic pathology inducers in cellular models.

The next phase (TRL 4) is the translation and verification of sensitivity in biological fluids (e.g., urine/saliva/serum/plasma/CSF) for diagnostic test development. Currently, the technology is already an advanced Drug Screening tool.

Type of innovation
Product innovation
Service/know how innovation
Description of innovative features / Competitive advantages

The competitive advantage is built on two fronts:

  1. Pharmaceutical Research (Drug Screening): The FRET CST sensor is a proven tool for drug discovery and screening, allowing for a rapid and quantitative real-time read-out of the efficacy of new molecules on pathological mechanisms in vitro.
  2. Future Diagnostics: The system offers the potential for non-invasive and low-cost screening (from urine/saliva/serum/plasma samples, once validated), overcoming the cost and invasiveness of current clinical diagnostics (PET/MRI, lumbar puncture).
  3. Early Detection: It detects Tau in its initial conformational form (CST), offering an earlier intervention window, which is crucial for new therapies.
  4. Sensitivity: The use of FRET ensures an extremely low limit of detection.

The technology is poised to transform the first-line diagnostics for Alzheimer's disease.

Reference market
Impacts on existing markets
Development stage
Feasibility
TRL
3
Advantages
New product/process/service/technology
Patentable technology
Yes
Patented technology
No
Publication of technology
Published
Technology validation/demonstration
Internal validation
Market positioning
Italian
European
International
Partner required
Enteprise
Public research center/university
Cooperation in national /european / international project

Information
For more information and/or to be put in contact with the Research Team, please contact the Project Manager:

Barbara Angelini - Project Manager
CNR - Unità Valorizzazione della Ricerca
Phone number 06.49932415
E-mail barbara.angelini@cnr.it