Molecole bioattive

Gliadin-specific intestinal T cell lines (iTCLs) represent a preclinical model of celiac disease (CD). They reproduce, in vitro, the Th1 CD4 T lymphocyte response activated by gluten peptides in the intestine of celiac patients, in vivo. They are generated from duodenal biopsies of celiac patients through cycles of in vitro stimulation with gluten. They can be expanded as polyclonal T cell lines or as single, highly specific, T cell clones and can be maintained in culture for extended periods.

The study of immune-mediated intestinal disorders, such as celiac disease, wheat allergy, non-celiac wheat sensitivity and inflammatory bowel diseases, requires experimental systems that are truly predictive for humans. Traditional animal models show significant limitations in reproducing human intestinal physiology and immune responses, hindering effective clinical translation.

The environment as well as the food production provide a number of both natural and synthetic compounds whose effects on human being as an organism have not yet been determined nor investigated.